I am a research associate, working on protein truncation and solubility studies. So anyone who could give me some handy tips on generating truncations in a complete ORF followed by cloning and expression studies would greatly appreciate.
How to design primers for generating truncated protein during PCR ?
Is it a common practice to check protein solubility while performing in vitro expression studies ? Also please let me know if there is any algorithm for protein solubility prediction?
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