western blotting question
Posted 30 October 2004 - 12:45 PM
1. Can I cast the gel one day before? (if it possible how can I preserve it?).
2. Or after SDS-PAGE can I perform blotting next day? (it possible how can I preserve the nitrocellulose fitler?)
Posted 01 November 2004 - 06:04 AM
Posted 01 November 2004 - 02:31 PM
You can easily keep your nitrocellulose membrane in blocking solution at 4 degrees over night, or block for 1h and leave in primary overnight shaking in the coldroom...
Posted 03 November 2004 - 10:25 AM