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Reference gene


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#1 molbio

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Posted 29 October 2004 - 05:43 AM

Hi,

I've been trying to treat cell lines with AZA and TSA and investigate whether expression is increased after treatment. The problem is that I can't find a stable reference gene that isn't affected by the treatment. Does anyone know of a good gene to use or has any ideas?

Thanks in advance

#2 rassen

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Posted 29 October 2004 - 08:01 AM

Aza and TSA are epigenetic reagent and may affect genes genome wide. They may also act through other pathways. So reference genes usually used for RT-PCR may also be affected such as beta-actin. I think using 18s and 28s rRNA gene as the control gene is OK.




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