I've been trying to treat cell lines with AZA and TSA and investigate whether expression is increased after treatment. The problem is that I can't find a stable reference gene that isn't affected by the treatment. Does anyone know of a good gene to use or has any ideas?
Thanks in advance
Submit your paper to J Biol Methods today!
1 reply to this topic
Posted 29 October 2004 - 08:01 AM
Aza and TSA are epigenetic reagent and may affect genes genome wide. They may also act through other pathways. So reference genes usually used for RT-PCR may also be affected such as beta-actin. I think using 18s and 28s rRNA gene as the control gene is OK.