edge effect in cell-based assays
Posted 28 October 2004 - 01:34 PM
I use 96/48-well plates for adherent cell culture, with about 25% confluency at seeding. I noticed that the cell distribution WITHIN each well is not uniform. There are more cells along the rim than at the center. I am not sure about the reason and couldn't think of a way to reduce this edge effect. Uniformity of cell seeding is very critical for me since I will be analyzing the wells individually by imaging to look at the effect of different compounds on cell growth.
Can somebody help me out? Thanks!
Posted 28 October 2004 - 03:46 PM
The edge effect cannot be avoided especially with 96well plate because the liquid tends to take a shap that has the least surface area so it will hang to the side wall and that is why there are fewer cells in the center. Try using plates with bigger wells if possible.
Posted 28 October 2004 - 04:17 PM
Posted 28 October 2004 - 09:23 PM
Posted 28 October 2004 - 10:08 PM
Edited by rassen, 28 October 2004 - 10:09 PM.