Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

DNA methylation


  • Please log in to reply
3 replies to this topic

#1 vkott

vkott

    member

  • Active Members
  • Pip
  • 9 posts
0
Neutral

Posted 28 October 2004 - 11:42 AM

Hi, I am having troubles with PCR after the DNA sulphitation (to study) DNA methylation. I think it is because of the low recuperation after the sulphite reaction.
Does somebody have experience with that? :lol:

#2 rassen

rassen

    Enthusiast

  • Active Members
  • PipPipPipPipPip
  • 49 posts
0
Neutral

Posted 31 October 2004 - 12:25 PM

I don't understand what you mean by "recuperation".

Many things contribute to failed PCR after DNA modification, such as unsuccessful modification, loss of DNA, poor primer design, not optimized PCR conditions... Please make your question clear and provide more details.

#3 vkott

vkott

    member

  • Active Members
  • Pip
  • 9 posts
0
Neutral

Posted 01 November 2004 - 05:51 AM

Sorry, I think it is loss of DNA during the reaction.

#4 rassen

rassen

    Enthusiast

  • Active Members
  • PipPipPipPipPip
  • 49 posts
0
Neutral

Posted 01 November 2004 - 03:54 PM

How did you purify your DNA after modification? Did you use a kit for the modification? DNA loss during purification is a problem but not the only problem for PCR failure. Try use some controls if you can get it such as good modified DNA or known working primers.




Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.