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[Glutinis]

Please give me your opinion. I just dont know why this kind of result happened for my PCR.

I did RT by using polyT+adapter, then PCR using the primer pair of a degenerative primer and adapter. Two bands were generated.

I eluted the cDNA out the two bands , respectively, and did nested PCR using the primer pair of another primer and the adapter for cDNA of each band. The result is, the cDNA having the same size with the tempelate cDNA was generated, even though one primer varies as compared to the previous PCR. The same thing happened when I chose another different primer and the adapter for PCR amplification on the cDNA band I eluted out previously. Seems like, as long as the adapter primer is there, no matter what the other primer is, the same size of PCR product will be generated. That is weird!