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Nucleic acid adherence to plastics


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#1 microbugger

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Posted 27 October 2004 - 03:45 PM

Hello all,

Does anyone know which types of plastic DNA, RNA, and proteins will NOT adhere to? I know PCR tubes are made of polypropylene. I am trying to minimize sample loss of DNA and RNA and have been told that polypropylene may cause adherence.

Thanks!

Edited by microbugger, 27 October 2004 - 03:45 PM.


#2 parasuis

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Posted 28 October 2004 - 03:26 AM

You could use siliconized tubes. Either buy them or make them yourself. We have a bottle of "repel silane" that's used for large SDS gels used for sequencing or what-have-you. Pour some in a tube (preferably under a fume hood!), close the tube, make sure the fluid gets everywhere, then pour the fluid into the next tube, repeat until there's no fluid left and you end up with a bunch of siliconized tubes. Leave open until dry.
Works great for eppendorfs, I haven't tried PCR tubes though.

Edited by parasuis, 28 October 2004 - 10:08 PM.


#3 bob1

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Posted 28 October 2004 - 04:59 PM

I was also going to suggest siliconised surfaces, but wasn't aware that plastics could be siliconised.

Sambrook et al.'s Molecular cloning: a laboratory manual should also have some ideas on this as well as methods of performing the treatments.




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