Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
- - - - -

Nucleic acid adherence to plastics

  • Please log in to reply
2 replies to this topic

#1 microbugger



  • Active Members
  • Pip
  • 6 posts

Posted 27 October 2004 - 03:45 PM

Hello all,

Does anyone know which types of plastic DNA, RNA, and proteins will NOT adhere to? I know PCR tubes are made of polypropylene. I am trying to minimize sample loss of DNA and RNA and have been told that polypropylene may cause adherence.


Edited by microbugger, 27 October 2004 - 03:45 PM.

#2 parasuis



  • Active Members
  • Pip
  • 5 posts

Posted 28 October 2004 - 03:26 AM

You could use siliconized tubes. Either buy them or make them yourself. We have a bottle of "repel silane" that's used for large SDS gels used for sequencing or what-have-you. Pour some in a tube (preferably under a fume hood!), close the tube, make sure the fluid gets everywhere, then pour the fluid into the next tube, repeat until there's no fluid left and you end up with a bunch of siliconized tubes. Leave open until dry.
Works great for eppendorfs, I haven't tried PCR tubes though.

Edited by parasuis, 28 October 2004 - 10:08 PM.

#3 bob1


    Thelymitra pulchella

  • Global Moderators
  • PipPipPipPipPipPipPipPipPipPip
  • 6,508 posts

Posted 28 October 2004 - 04:59 PM

I was also going to suggest siliconised surfaces, but wasn't aware that plastics could be siliconised.

Sambrook et al.'s Molecular cloning: a laboratory manual should also have some ideas on this as well as methods of performing the treatments.

Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.