One plasmid contains two AvrII sites. I can not digest it by AvrII. But this plasmid can be digested by BamHI, BssHII, SacI, PspXI. The AvrII is new stock just bought from NEB, and Buffer2 was used. Does somebody else has experience about this enzyme?
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#2
richel
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Posted 23 November 2004 - 05:00 AM
just wondered if you found the answer to the AvrII problem you were having, as having a similar problem myself?
#3
suds
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Posted 23 November 2004 - 05:57 AM
I used AvrII recently, bought from NEB. I didn't have any problems with it.
#4
richel
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Posted 25 November 2004 - 02:20 PM
This was fresh from NEB as well, but i have found i need to add an excess of enzyme and leave overnight to digest completely...problem solved 
