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question about UV melting experiments


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#1 wjchxl

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Posted 25 October 2004 - 06:47 PM

[I am not sure if this message could be posted here or not. If not, sorry for any inconvenience.]

I am going to do UV melting experiments to determine Tm of dsRNA. I am not sure how much materials I should use. Could anyone tell me what A260 value is appropriate (accurate) for UV melting experiments? Thanks!





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