Hi everyone,
I cotransfect my cells with EGFP vector and promoter vector with luciferase reporter in a 1:100 ratio. I measure the luciferase activity using a luminometer and the fluoresence using a fluorometer. To correct for transfection efficiency I take the highest fluoresence reading and divide that number by all other fluoresence readings to get a correction factor. I then multiply the luminesence measurements by this correction factor and plot the data. Is this a valid normalization technique?? What should I be doing to correct for transfection efficiency??
Thanks in advance for your replies.
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