Posted 06 April 2002 - 02:48 AM
Posted 06 April 2002 - 03:05 AM
If you have primers on the both sides (for ex. before BamHI and after HindIII) You can make PCR. If after PCR you see line of DNA and size is similar to you cloned fragment and after transformation you have no clones then you have not problem with ligation reaction. The matter in toxity of cloned fragment.
Posted 20 June 2002 - 06:10 PM
i have the same problem,can you help me ?
Posted 22 June 2002 - 11:12 AM
Posted 24 June 2002 - 06:15 AM
increase the insert /vector ratio
Posted 24 June 2002 - 06:17 AM
increase insert/vector ratio
purify both insert and vector before ligation