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Precipitation with TCA and Na deoxycholate


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#1 Maud

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Posted 25 October 2004 - 05:05 AM

I'm working on extracellular proteins of bacterium, by bidimensionnal electrophoresis.
To recover these proteins, I precipitate the culture medium filtered, with TCA. I saw that in certain protocols, deoxycholate was added before precipitation. I tested this protocol and deoxycholate enabled me to recover a greater quantity of proteins. However, I don't know the role of sodium deoxycholate. Can you tell me what is its role?

Thank you very much.

#2 Maud

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Posted 26 October 2004 - 01:47 AM

Nobody know?
I have just call SIGMA, and they could not answer me.
Please help me.

#3 Simonsays

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Posted 26 October 2004 - 10:46 AM

I personaly use deoxycholate in lysis buffer to release membrane proteins... it is a mild detergent...

Simon

#4 Sprag

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Posted 16 November 2004 - 08:35 AM

deoxycholate binds to the protein and increases the interaction with the TCA. You usually add it for precipitation of very small amounts of protein with TCA..

#5 Maud

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Posted 19 November 2004 - 12:02 AM

Thank you for your answer!




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