Precipitation with TCA and Na deoxycholate
Posted 25 October 2004 - 05:05 AM
To recover these proteins, I precipitate the culture medium filtered, with TCA. I saw that in certain protocols, deoxycholate was added before precipitation. I tested this protocol and deoxycholate enabled me to recover a greater quantity of proteins. However, I don't know the role of sodium deoxycholate. Can you tell me what is its role?
Thank you very much.
Posted 26 October 2004 - 01:47 AM
I have just call SIGMA, and they could not answer me.
Please help me.
Posted 26 October 2004 - 10:46 AM
Posted 16 November 2004 - 08:35 AM