4 replies to this topic

[Maud]

I'm working on extracellular proteins of bacterium, by bidimensionnal electrophoresis.
To recover these proteins, I precipitate the culture medium filtered, with TCA. I saw that in certain protocols,  deoxycholate was added before precipitation.  I tested this protocol and deoxycholate enabled me to recover a greater quantity of proteins.  However, I don't know the role of sodium deoxycholate.  Can you tell me what is its role?

Thank you very much.

[Maud]

Nobody know?
I have just call SIGMA, and they could not answer me.
Please help me.

[Simonsays]

I personaly use deoxycholate in lysis buffer to release membrane proteins... it is a mild detergent...

Simon

[Sprag]

deoxycholate binds to the protein and increases the interaction with the TCA. You usually add it for precipitation of very small amounts of protein with TCA..

[Maud]

Thank you for your answer!