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RT-PCR


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#1 egrego

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Posted 24 October 2004 - 07:35 AM

I have to denature a double strand RNA to be used in RT-PCR. I have read that the methilmercuric hydroxide can be used but I have not been able to find it. Have some of you experienced the same problem ? How have you resolved it ?

#2 GT(N)T

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Posted 25 October 2004 - 10:58 AM

Methylmercury hydroxide can be obtained from Alfa Aesar.

Careful, that is just about the nastiest stuff I've ever worked with.

#3 egrego

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Posted 26 October 2004 - 01:44 AM

I try to obtein the methylmercury from Alfa Aesar but they told me that is not possible to send it in Italy




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