I have to denature a double strand RNA to be used in RT-PCR. I have read that the methilmercuric hydroxide can be used but I have not been able to find it. Have some of you experienced the same problem ? How have you resolved it ?
RT-PCR
Started by egrego, Oct 24 2004 07:35 AM
2 replies to this topic
#1
Posted 24 October 2004 - 07:35 AM
#2
Posted 25 October 2004 - 10:58 AM
Methylmercury hydroxide can be obtained from Alfa Aesar.
Careful, that is just about the nastiest stuff I've ever worked with.
Careful, that is just about the nastiest stuff I've ever worked with.
#3
Posted 26 October 2004 - 01:44 AM
I try to obtein the methylmercury from Alfa Aesar but they told me that is not possible to send it in Italy













