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[manojupreti]

Hello Friends
I have some Tn10 derivative trasposon in plasmid vehicle cloned in E.coli. Example of them are - Derivative 105,marker in transposon is CamR and venicle is plasmid pnK2284,and deirivative 108 ,Markers in transposon is CAmr ,Plac ,plasmid vehicle is pNk2887.
I want use them for random mutagenesis in Pseudomonas.
Can you please suggest the protocol for mutagenesis using these transposon.
Regards
Manoj Kumar,Ph.D.[COLOR=blue]