Is REVERSE TRANSCRIPTASE-PCR and REAL-TIME PCR the same thing?
If not, what dummy decided to use RT-PCR as the acrinym for both?
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Posted 22 October 2004 - 04:54 AM
no it isn't.
RT-PCR as reverse transcriptase PCR means a combination of first reversely transcribing RNA into cDNA, followed by a "normal" PCR. This can be done in seperate steps, but "one-tube" reaction mixes are also available.
RT-PCR as real-time PCR is the quantitative detection (by increase in flourescence) of templates with either Lightcycler or Taqman methods. I.e. you can watch when your PCR-reaction is starting or in the exponential phase...
the template used for real-time PCR can be cDNA generated by reverse transcription, though.
why the two have the same acronyms is purely coincidental. real-time PCR is often abreviated as qRT-PCR, meaning quantitative real-time PCR.
BTW, RT-PCR isn't room-temperature PCR either, as a student around here asked some time ago!
mike
RT-PCR as reverse transcriptase PCR means a combination of first reversely transcribing RNA into cDNA, followed by a "normal" PCR. This can be done in seperate steps, but "one-tube" reaction mixes are also available.
RT-PCR as real-time PCR is the quantitative detection (by increase in flourescence) of templates with either Lightcycler or Taqman methods. I.e. you can watch when your PCR-reaction is starting or in the exponential phase...
the template used for real-time PCR can be cDNA generated by reverse transcription, though.
why the two have the same acronyms is purely coincidental. real-time PCR is often abreviated as qRT-PCR, meaning quantitative real-time PCR.
BTW, RT-PCR isn't room-temperature PCR either, as a student around here asked some time ago!
mike
--- He who finds typos may keep them! ---
