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Luciferase assay


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#1 RLeah

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Posted 21 October 2004 - 11:18 AM

Does anyone know what "relative luciferase units" really measure? Are numbers like, say, 6.2, if they are 80x over background, considered "good" (i.e. valid) numbers? I see people reporting numbers in the 100,000 range. Are these the same units? Any clarification would be appreciated.
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Randi

#2 pcrman

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Posted 21 October 2004 - 04:30 PM

I usually use two control vectors to be transfected with my experimental vector, one is beta-gal vector and the other is SV40 promoter driven luc vector. I cotransfect beta-gal and experimental vector, and transfect SV40 in a separate well, then measure beta-gal and luc activity, then correct luc activity to beta-gal activity to control transfection efficiency, then divide the corrected luc activity by the luc activity from SV40 transfected cells and get relative luc unit (RLU).

There are also other formula for the calculation.

#3 zienpiggie

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Posted 24 October 2004 - 10:46 PM

I wonder what's the difference between relative light unit and relative luciferase unit?




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