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How to PCR GC-rich inserts


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#1 anonymous

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Posted 24 October 2001 - 09:00 PM

I'm having problems trying to pcr two GC-rich transcription factors (Twist and Dermo). Would someone please offer me some suggestions. Thank you!

#2 anonymous

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Posted 25 October 2001 - 09:00 PM

Have you tried Betain (Henke-W et al.)? But be careful the concentration mentioned there is to high.

#3 anonymous

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Posted 25 October 2001 - 09:00 PM

there are a number of enhancer adjuvants available to circumvent the GC rich area. betaine is certainly one of them. you can also you glycerol, gelatin, DMSO. check out the following web site. the researcher whocreated this site did an excellent job!

[url="http://info.med.yale.edu/genetics/ward/tavi/PCR.html"][url]http://info.med.yale.edu/genetics/ward/tavi/PCR.html[/url][/url]






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