Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

centrifuging


  • Please log in to reply
1 reply to this topic

#1 jasonrc

jasonrc

    member

  • Members
  • Pip
  • 4 posts
0
Neutral

Posted 20 October 2004 - 09:56 AM

Hi,

Our lab protocol for obtaining protein from rat brain is:

1. Perfuse with saline
2. Remove brain
3. Sonicate in sample buffer for 15 sec.
4. Centrifuge at 14,000 rpm X 15 min.
5. Obtain supernatant discard solid

My question is this: During centrifuging, will transmembrane proteins, such as receptors be lost, or are they realeased during sonicating?

Thanks,

Jason

#2 quol

quol

    member

  • Active Members
  • Pip
  • 8 posts
0
Neutral

Posted 20 October 2004 - 11:42 PM

During sonication membranes break releasing the content of the cells and organells and they close again. No, you don't release transmembrane proteins, they will stay in the pellet.




Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.