Our lab protocol for obtaining protein from rat brain is:
1. Perfuse with saline
2. Remove brain
3. Sonicate in sample buffer for 15 sec.
4. Centrifuge at 14,000 rpm X 15 min.
5. Obtain supernatant discard solid
My question is this: During centrifuging, will transmembrane proteins, such as receptors be lost, or are they realeased during sonicating?
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