Cell lysis for peptide binding assay
Posted 19 October 2004 - 03:52 PM
I'm doing a peptide binding assay where I need to lysis cells and expose a chemical in the cells after initial binding. I don't really need to reserve any proteins as I'm interested in the chemicals and it should be pretty tough (well, at least the chemist told me so). So does that mean I can just use simple detergent to do the job? Can anyone give me some suggestion please?
Posted 20 October 2004 - 06:24 AM
Posted 22 October 2004 - 12:40 AM
Posted 23 October 2004 - 01:25 AM