Im doing a cell proliferation assay using MCF-7 cells and estradiol. I've already done 2, which worked pretty darn well
, but they used 1nM of E2. now, i have to use the amount of E2 that produces the most cell proliferation.... my supervisor suggested 100nM.... but i've seen a paper that said an amount that high might cause cell death. i can't quote that paper because it uses other drugs involved in that assay. i'm under the impression, that the max rate is achieved at 10nM... but i don't know where this is written.
can someone please tell me if this is the right amount.... and where i can get the reference from?
Edited by vetticus3, 17 October 2004 - 05:08 PM.