2 replies to this topic

[vetticus3]

Im doing a cell proliferation assay using MCF-7 cells and estradiol.  I've already done 2, which worked pretty darn well   , but they used 1nM of E2.  now, i have to use the amount of E2 that produces the most cell proliferation....  my supervisor suggested 100nM.... but i've seen a paper that said an amount that high might cause cell death.  i can't quote that paper because it uses other drugs involved in that assay.  i'm under the impression, that the max rate is achieved at 10nM...  but i don't know where this is written.
can someone please tell me if this is the right amount.... and where i can get the reference from?

thanks heaps
vetticus

Edited by vetticus3, 17 October 2004 - 05:08 PM.

[zienpiggie]

why not try both concentrations ?

[vetticus3]

THANKYOU!!!!
i'm actually going to do a dose dependent study on estrogen.  using several concentrations, 5,10, 15 etc.  probably up to 50.
thanks for the idea.