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Gene Synthesis


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2 replies to this topic

#1 iriana

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Posted 16 October 2004 - 12:30 AM

I am a research associate and about to start with my project work. I would be working on gene synthesis so any guys who could help me with this would greatly appreciate. I have my protein sequences . What I need is to generate a  DNA sequence optimized for expression system of choice.

Which is the most  appropriate method for this- PCR assembly or LCR coupled to PCR ?

Also please let me know if there is any software available for oligo design?

#2 chandima

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Posted 17 October 2004 - 11:01 PM

hi
Primer3 would help
http://www-genome.wi...er/primer3.html
good luck
chandima

#3 laojun

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Posted 18 October 2004 - 01:05 PM

http://www.changbios...imo/primoo.html

There is also an academica software. It is probably published in Bioinformatics.




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