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measuring apoptosis after treatment of cells with ZVAD-FMK


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#1 christii

christii

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Posted 15 October 2004 - 09:39 AM

Hi,
I have been culturing cells with a compound that seems to increase cell death.
To see whether it might be doing this by increasing apoptosis i was going to treat cells with ZVAD-FMK after pre-incubation with my compound. Which method of apoptosis detection would be easiest and most precise to use? I don't want to use TUNEL assay as people in lab have had huge problems with it.
Thank you for your help!




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