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cell fixation


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5 replies to this topic

#1 geness

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Posted 15 October 2004 - 06:48 AM

hi,everyone

I'd like to observe the cells using the Laser Scanning Confocal

Microscopy (LSCM), but I do not know how to dispose my cells before the

observation, that is to say, I want to know how to fix my cells effectively

so that the detectedcells could be observed much more truely.

Would you mind sharing with me if you know about it happenly?

Thanks a lot!

#2 leahf

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Posted 17 October 2004 - 04:25 AM

Hi
What cells are you using?
we fix bacteria in 4% formaldehyde for 1 to 24 hours, works just fine for LSCM

#3 geness

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Posted 18 October 2004 - 02:31 AM

hi,leahf

The cells used are HEK 293T cell lines.

#4 sebwood

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Posted 03 December 2004 - 01:54 AM

hi

u can use PFA 3% + gluta 0.5% 10 min at 37C
rince with PBS or Tris 3*10min
u can store them 1 month at 4C.

#5 anilkumarpr

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Posted 03 December 2004 - 09:31 AM

Hi Geness

4 % paraformaldehyde in PBS will good for fixing cells.

Anil

#6 geness

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Posted 09 January 2005 - 06:20 AM

hi,

Thanks a lot!

I believed that 4 % paraformaldehyde in PBS is good for fixing mammalian

cells.

But I'd like to know where I could get the manufacturer's instruction

about detailed fixing cells for Confocal detection.

If you know,would you mind sharing with me ? Thank you very much!




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