Posted 15 October 2004 - 05:11 AM
I try to reveal the MAPK P38 (phosphorylated) with cell signaling antibodies but no band appears. P38 antibody is at 1/1000.
My procedures are OK with other antibodies. Did someone have troubles with this antibody ?? How many micrograms of protein (or cell lysat) do you load per lane ?
Posted 22 November 2004 - 05:49 PM
I also had the same trouble with cell signalling antibody.
Did you repeat the experiment?
Let me know if you have found out a way
Posted 22 November 2004 - 10:53 PM
Posted 24 November 2004 - 05:35 AM
Posted 21 December 2004 - 05:51 AM
Posted 04 February 2005 - 02:52 PM