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Suspension Culture


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4 replies to this topic

#1 superleo

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Posted 14 October 2004 - 11:37 PM

My suspension cell culture is growing remarkably rapidly. The problem with it is that it forms a cluster, rendering it diffcult to perform cell count. So, is there any protocol that can transform it into an adherent one?
Thank you all in advance

#2 zienpiggie

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Posted 16 October 2004 - 12:36 AM

Hello, I don't really have experience in converting suspension to adherent, but I have several ideas. To get rid of the clumping before cell count, how about trypsinizing it?

And to convert from suspension to adherent, how about coating your wells, like with collagen or fibronectin or those substances that may enhance adherence?

#3 superleo

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Posted 17 October 2004 - 06:33 PM

Thanks indeed ziepiggie for your good ideas. I will try them out. ;)

#4 sea_liu

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Posted 06 December 2004 - 08:03 AM

i don't know the purpose of your cell suspension culture method, does the

cells grows in suspension condition such as some tumor cells ,or you had
the cells suspended?

#5 Indigo

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Posted 16 January 2005 - 01:29 AM

Hai, I usually give a wash with PBS-EDTA to prevent the cells from clumping every alternate days and before counting and it works nicely for me.




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