Hi,
I m using one protocol for isolating protein from liver tissue of mouse.
Tissue is homogenized in hypotonic buffer (10mM tris, 5mM EDTA, 150mM NaCl, 10% Sucrose with protease inhibitor). Then, 1/10th volume of 10x buffer (10% Triton X100, 1% SDS, 5% Sodium deoxycholate). Biochemical Pharmacology 66 (2003) 393-403
The problem I m facing is this, that the proteins when run on SDS PAGE give bands only in high molecular weight range; the bands in low molecular weight are very faint.
Does anybody have any solution for this?
Thanks.
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