I'm new to this forum. thanks for this great resource.
I'm working on dna methylation and after repeated trials my pcr doesnt work. I am using chemicon kit. I am using the dna extracted from the fresh tissue samples.
the wild type unmodified dna samples work good but the modified dna with the methylation specific primers doesnt work.
one thing I noticed is the purity ratios on the spectrometer reading are shown as 20 and sometimes 30. its wiered.
can anyone suggest the best purification method and is it better to purify before or after midification.
appreciate ur help.
Edited by pcrman, 11 October 2004 - 09:40 PM.