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FAM and other green flourescent dyes

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#1 leahf



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Posted 11 October 2004 - 04:33 AM

Hi All
Has anyone any experience with oligos modified by FAM? i have used such an oligo for staining bacteria for FISH, but got almost no signal (the same probe marked with cy3 gave a very good signal). Never having worked with FAM before, i don't know wheter to blame the manufacturer or my protocol - is there a way to check the quality of the probe?
does anybody have any experience with other dyes that that excite in the blue (480) and flourescence in the green (around 530) - I have heard of Alexa, Oregon and Cy2 dyes, but they are rather expensive and would like to get advice on which to order beforehand.
Thank You!

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