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Chaotropic Salts for DNA Extraction


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#1 BNing

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Posted 09 May 2002 - 07:29 AM

Hi,

I have been successfully extracting plasmid fragments from 2mls of FCS by using Qiagens Gel Extraction Kit by adding the required 6mls (3vols) of buffer QG. This is proving expensive and I would like to replace the QG with a another chaotropic salt solution. Has anyone had success using KI solutions with the kit? Can anyone suggest another salt.

Thanks for your help,

Benjamin Ning.


#2 BNing

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Posted 09 May 2002 - 07:38 AM

Sorry. I meant NaI in the last post.

Ben.


#3 milanoj

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Posted 09 May 2002 - 09:50 AM

You can try Guanidine HCl. It's a pretty potent chaotrope.




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