Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
- - - - -

Cell density question

  • Please log in to reply
No replies to this topic

#1 Neal



  • Members
  • Pip
  • 1 posts

Posted 28 April 2022 - 12:39 PM

Hi Guys-

I'm new to Tissue culture and have a basic question.

I have 2 cell lines: Line X doubling time=50 hr and Line Y doubling time=37 hrs.


My question is that I want to do a 5 day tmnt with a drug and harvest all the samples at the same time. So for example, I'll start my 5 day tmnt. on a Fri etc--see below. And harvest all the samples on the following Wed. My issue is that I need to maintain similar density for ALL samples--how do I calculate what density I should plate all the tmnt samples so that when I harvest cells on Wed--the data won't be confounded by cell density? Thanks so much!!!!

          Day Tmnt.Time Start tmnt Fri d0 5d 12pm Sun d2 3d 12pm Tues d4 1d 12pm Wed d5 3hr 9am Wed d5 Ohr 12pm        


Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.