I'm new to Tissue culture and have a basic question.
I have 2 cell lines: Line X doubling time=50 hr and Line Y doubling time=37 hrs.
My question is that I want to do a 5 day tmnt with a drug and harvest all the samples at the same time. So for example, I'll start my 5 day tmnt. on a Fri etc--see below. And harvest all the samples on the following Wed. My issue is that I need to maintain similar density for ALL samples--how do I calculate what density I should plate all the tmnt samples so that when I harvest cells on Wed--the data won't be confounded by cell density? Thanks so much!!!!Day Tmnt.Time Start tmnt Fri d0 5d 12pm Sun d2 3d 12pm Tues d4 1d 12pm Wed d5 3hr 9am Wed d5 Ohr 12pm