I designed primers using methprimer.
1. Before using methprimer. I BLAST my promoter sequence to confirm its from my gene of interest, and it was
2. I than put he promoter sequence on methprimer and it generated primers
3. When I blasted the primers (methylated primers and unmethylated primers), the results dont match my gene of interest.
Is there anything Im doing wrong? I thought the methylated primer sequence would match my gene using BLAST