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BLAST primers

methylation primers

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#1 SF_HK



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Posted 15 July 2021 - 09:55 PM



I designed primers using methprimer.


1. Before using methprimer. I BLAST my promoter sequence to confirm its from my gene of interest, and it was

2. I than put he promoter sequence on methprimer and it generated primers

3. When I blasted the primers (methylated primers and unmethylated primers), the results dont match my gene of interest.


Is there anything Im doing wrong? I thought the methylated primer sequence would match my gene using BLAST


Thank you


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