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stable cell line from lentvirus

no mammalian drug select

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#1 SF_HK

SF_HK

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Posted 25 May 2021 - 10:45 PM

Hi,

 

I have to transduce a mammalian cell line with ready-to use lentivirus particles. But the vector does not have a a drug selection marker for mammalian selection. 

 

In such a case, how are cells selected for generation of stable cell line following transduction using lentiparticles in mammalian cells?

 

Ive generated several stable cell lines in the past but all containing vectors with mamalian antibiotic selection.

 

Thank you

SF






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