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methylation specific PCR

gel electrophoresis

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#1 SF_HK

SF_HK

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Posted 23 May 2021 - 07:50 PM

Hi,

 

Im new to methylation specific PCR (MSP)and would appreciate all the advice

 

I plan to do end-point MSP followed by gel electrophoresis.

 

I have a few doubts that I wanted to clear:

 

1. What is the gel loading control. Will it be actin as well?

2. I have also ordered control DNA (bilsulphite methylated/unmethylated DNA, and also non bisulphite converted unmethylated DNA). So I can check the specificity of my primers by using these control DNA right? If I do not get bands it means I need to re-design my primers?

3. I should also run a negative control (no template).

 

Many thanks

SF







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