Im a full time working mom and its difficult for me spend more than 2 hours in lab on Saturday and Sundays. Im just thinking of how I can use my time efficiently.
1. protein SDS gels can be prepared in advance
2. collecting protein from cell lines takes time. Has anyone just washed their cell with PBS and froze the cells in -80deg with RIPA buffer over the weekend and continued on a Monday?
3. running 3 full 96-well plates on qPCR (sybrgreen). Can I prepare all the 3 plates at once and cover them with foil in fridge. I can just load them everytime a qPCR round is finished?
4. any other tips for cell culture or other in vitro assays, will be appreciated?