5ul PCR reactions with two sets of primers, the lanes should have up to 4 bands. The PCR buffer is "ready to load"
1.5% agarose gel made with 1x TAE buffer, run at 80V in 1x TAE buffer
I dilute the samples 1:3 with 1x loading buffer and load 1ul of the diluted sample (I often get smeared bands otherwise)
Ladders are 1kb and 50bp ladders