Getting cultures to specific cfu
Posted 19 March 2021 - 02:49 AM
Posted 19 March 2021 - 05:35 AM
Suggest you develop a standard curve - OD v. cfu. Hematcytomer counts are a pain - esp. for bacteria
Posted 19 March 2021 - 05:57 AM
Posted 19 March 2021 - 08:27 AM
Posted 20 March 2021 - 04:33 AM
Bacteria are hard to see and reliably count using a hemacytometer. Look for a used Klett meter.
Posted 20 March 2021 - 10:35 AM
Posted 20 March 2021 - 11:00 AM
No - take an OD AND a cfu count at various dilutions of a culture and plot OD v. log cfu. Using that plot - you can roughly estimate count colony count based on OD.