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eliminating albumin from samples for western blotting ?.


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#1 jackyjoy123

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Posted 20 February 2021 - 05:53 AM

Hello,

 

 

I am starting to study a protein which is secreted from cultured mammalian cell
lines. I need to concentrate the conditioned medium to do some Westerns. The
problem is that this way I have so much albumin from serum in my samples which
makes a "blob" in the gel... How could I get rid of it? Is there any affinity
chromatography I can use to absorb albumin?
my protein is about 30 KDa and pI is 6.66; albumin is 68KDa and pI is 5.8.

 

 

thanks

jackyjoy



#2 mdfenko

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Posted 21 February 2021 - 10:37 PM

Cytiva sells an albumin and IgG removal kit. You can also use blue dextran agarose to remove albumin i think this is what the kit contains). You can see a publication from cytiva at this address: https://cdn.cytivali...6&assetid=12856


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