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Liquid nitrogen protein extraction ?.


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#1 jackyjoy123

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Posted 01 January 2021 - 03:49 AM

Hello,

 

 

After grinding my sample into powder using liquid nitrogen, is it okay if I transport the powder on ice (journey cost about 30 minutes) before I could place them into -20C/-80C? I am worried that during the transportation period the powder will thaw and cause protein degradation.

 

 

thanks

jackyjoy



#2 mdfenko

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Posted 02 January 2021 - 08:23 PM

Should be okay, better if dry ice


talent does what it can
genius does what it must
i used to do what i got paid to do


#3 christinaroberts

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Posted 04 March 2021 - 05:53 AM

 This is how you could do it by blending protein samples with liquid nitrogen until they freeze and become brittle. The samples can then be ground with a mortar and pestle. Grinding involves low risk to the integrity of the proteins, but liquid nitrogen can be dangerous. Grinding in liquid nitrogen is a great technique for difficult samples, such as plant and fungi samples.






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