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Storing plasmid at 4 degrees C ?.


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#1 alexsunny123

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Posted 29 December 2020 - 02:34 AM

Hello,

 

I have plasmid prepared from maxi prep, and i am aliquoting them into 25ul portions and keeping them at -20C. I am using them for transfection, so i am planning to take each portion out for us and then keep it at 4 degrees C for a few days as I do the transfection. How long can the plasmid be kept at 4degrees C before it starts to breakdown?

 

 

thanks

alexsunny



#2 mdfenko

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Posted 30 December 2020 - 06:21 PM

If it’s stored in water then i would defrost and use immediately. If in buffer then you may be able to store it, refrigerated, for a few days.


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#3 alexsunny123

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Posted 31 December 2020 - 11:54 PM

Hello,

 

I have plasmid prepared from maxi prep, and i am aliquoting them into 25ul portions and keeping them at -20C. I am using them for transfection, so i am planning to take each portion out for us and then keep it at 4 degrees C for a few days as I do the transfection. How long can the plasmid be kept at 4degrees C before it starts to breakdown?

 

 

thanks

alexsunny

 

 

thanks my issue has been fixed.






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