Greetings to everyone!
I'm a university student working on an assignment with another classmate and I'm not sure I have identified the complimentary strand in the 5'-3' direction for the DNA fragment below.
My original answer is: TGACTAAGCTACGTTACCGCATGTAGGCATGCATCTCGATAGCTACGTTAGCCACTAGATTCA
However, I have a classmate that has a different answer: ACTTAGATCACCGATTGCATCGATAGCTCTACGTACGGATGTACGCCATTGCATCGAATCAGT
He says that my answer is not correct because it is in the 3'-5' direction. Can someone please help me figure out who came up with the right complimentary strand in the 5'-3' direction?
3’ – ACTGATTCGATGCAATGGCGTACATCCGTACGTAGAGCTATCGATGCAATCGGTGATCTAAGT – 5’
5’ – CTACGTT – 3’
3’ – GTGATCT – 5’