I am trying to do a very standard subcloning and getting results such as:
after ligating, transforming, and getting colonies, the mini-preps show no insert in the diagnostic cut (even though the ratio of colonies compared to the background plate looks great)
But when I sequence these mini's the insert is there! It can't be the enzymes because, I have replaced them. Also, they put the insert there in the first place.
Has anyone had problems like this before?? Any ideas??
Edited by yhafezi, 06 October 2004 - 12:09 PM.