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Evaluation of cell migration assay data - which option to use?

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#1 Biochemistica



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Posted 01 September 2020 - 05:18 AM

I have data from a Transwell cell migration assay that I need to evaluate statistically. The data that I obtained directly from the assay are the total numbers (#) of migrated cells for each replicate and condition (control vs treatment with different substances).


What I want to know is whether there is a significant difference between the control (= untreated cells) and the experimental set-ups where the cells were treated with substance 1 or substance 2?


Now, there are various possible ways of evaluating the data. In order to find out whether there a significant difference between the control and the treated cells in respect to migration, I could do the following types of comparisons:


a)  total # of migrated cells under the different conditions
b )  % of migrated cells relative to total # of seeded cells
c)  % increase in # of migrated cells vs control mean
d)  fold change of # of migrated cells vs control mean


Depending on which data sets I use, I would get different results from the statistical analysis. Which type of comparison would you say is best in general and/or best for statistical analysis and why?

Edited by Biochemistica, 01 September 2020 - 05:18 AM.

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