I have data from a Transwell cell migration assay that I need to evaluate statistically. The data that I obtained directly from the assay are the total numbers (#) of migrated cells for each replicate and condition (control vs treatment with different substances).
What I want to know is whether there is a significant difference between the control (= untreated cells) and the experimental set-ups where the cells were treated with substance 1 or substance 2?
Now, there are various possible ways of evaluating the data. In order to find out whether there a significant difference between the control and the treated cells in respect to migration, I could do the following types of comparisons:
a) total # of migrated cells under the different conditions
b ) % of migrated cells relative to total # of seeded cells
c) % increase in # of migrated cells vs control mean
d) fold change of # of migrated cells vs control mean
Depending on which data sets I use, I would get different results from the statistical analysis. Which type of comparison would you say is best in general and/or best for statistical analysis and why?
Edited by Biochemistica, 01 September 2020 - 05:18 AM.