Posted 05 October 2004 - 08:46 AM
Looking forward to hearing from somebody.
Posted 05 October 2004 - 09:05 AM
maybe you could consider a strep-tag as an alternative, but you could go with the flag-tag first.
often protein cristallographers use the his-tag in variant sizes (4x - 10x His), but the proteins is denatured in the process of elution and has to be refolded for structure determination.
Edited by jadefalcon, 05 October 2004 - 09:05 AM.