Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
- - - - -

Re-PCR on a gel purified PCR product resulted in smear. Why?

  • Please log in to reply
No replies to this topic

#1 Curtis


    Metaller Scientist

  • Global Moderators
  • PipPipPipPipPipPipPipPipPipPip
  • 1,201 posts

Posted 27 July 2020 - 08:34 PM

I got a faint 4.3 kb pcr product on gel so I decided to purify and use it as template for a 2nd pcr with the same primers and machine program. Instead I ended up with a huge smear, although my own band of interest was visible at the top. I purified it but I want to know why I got a smear? was the annealing temp too low or the template was too much?

Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.