How can I determine the Protein concentration from its extinction coefficient. If the extinction coefficient is 14480. What will the procedure to determine its concentraion.
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#2
mdfenko
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Posted 20 July 2020 - 05:54 PM
A=ecl
A=absorbance
e=extinction coefficient
c=molar concentration
l=path length
rearranging to solve for c:
c=A/el
however, you can ignore l if it is the same for all measurements
therefore
molar concentration = absorbance/extinction coefficient
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#3
samita
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Posted 21 July 2020 - 06:18 AM
Thanks for your reply.
it will be molar concentration means Moles/litre (mM/ml and uM/uL) and to convert it into mg/ml we can simply multiple its molecular weight in daltons (grams/mole). The unit will be grams/lit (mg/ml and ug/uL). Will it be like this.
#4
mdfenko
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Posted 21 July 2020 - 05:54 PM
Yes
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#5
mdfenko
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Posted 22 July 2020 - 08:28 AM
Also, pay attention to the decimal point on absorbance readings (and on the extinction coefficient)
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