How can I determine the Protein concentration from its extinction coefficient. If the extinction coefficient is 14480. What will the procedure to determine its concentraion.
Submit your paper to J Biol Methods today!
4 replies to this topic
#2
mdfenko
-
- Active Members
-
- 3,361 posts
an elder emeritus
216
Excellent
Excellent
Posted 20 July 2020 - 05:54 PM
A=ecl
A=absorbance
e=extinction coefficient
c=molar concentration
l=path length
rearranging to solve for c:
c=A/el
however, you can ignore l if it is the same for all measurements
therefore
molar concentration = absorbance/extinction coefficient
talent does what it can
genius does what it must
i used to do what i got paid to do
#3
samita
-
- Active Members
-
- 153 posts
Veteran
6
Neutral
Neutral
Posted 21 July 2020 - 06:18 AM
Thanks for your reply.
it will be molar concentration means Moles/litre (mM/ml and uM/uL) and to convert it into mg/ml we can simply multiple its molecular weight in daltons (grams/mole). The unit will be grams/lit (mg/ml and ug/uL). Will it be like this.
#4
mdfenko
-
- Active Members
-
- 3,361 posts
an elder emeritus
216
Excellent
Excellent
Posted 21 July 2020 - 05:54 PM
Yes
talent does what it can
genius does what it must
i used to do what i got paid to do
#5
mdfenko
-
- Active Members
-
- 3,361 posts
an elder emeritus
216
Excellent
Excellent
Posted 22 July 2020 - 08:28 AM
Also, pay attention to the decimal point on absorbance readings (and on the extinction coefficient)
talent does what it can
genius does what it must
i used to do what i got paid to do
