Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

Protein concentration for ext. coefficient


  • Please log in to reply
4 replies to this topic

#1 samita

samita

    Veteran

  • Active Members
  • PipPipPipPipPipPipPipPipPipPip
  • 153 posts
6
Neutral

Posted 20 July 2020 - 01:30 AM

How can I determine the Protein concentration from its extinction coefficient. If the extinction coefficient is 14480. What will the procedure to determine its concentraion.

 

 



#2 mdfenko

mdfenko

    an elder emeritus

  • Active Members
  • PipPipPipPipPipPipPipPipPipPip
  • 3,361 posts
216
Excellent

Posted 20 July 2020 - 05:54 PM

A=ecl

 

A=absorbance

 

e=extinction coefficient

 

c=molar concentration

 

l=path length

 

rearranging to solve for c:

 

c=A/el

 

however, you can ignore l if it is the same for all measurements

 

therefore

 

molar concentration = absorbance/extinction coefficient


talent does what it can
genius does what it must
i used to do what i got paid to do


#3 samita

samita

    Veteran

  • Active Members
  • PipPipPipPipPipPipPipPipPipPip
  • 153 posts
6
Neutral

Posted 21 July 2020 - 06:18 AM

Thanks for your reply.

it will be molar concentration means Moles/litre (mM/ml and uM/uL) and to convert it into mg/ml we can simply multiple its molecular weight in daltons (grams/mole). The unit will be grams/lit (mg/ml and ug/uL). Will it be like this.



#4 mdfenko

mdfenko

    an elder emeritus

  • Active Members
  • PipPipPipPipPipPipPipPipPipPip
  • 3,361 posts
216
Excellent

Posted 21 July 2020 - 05:54 PM

Yes


talent does what it can
genius does what it must
i used to do what i got paid to do


#5 mdfenko

mdfenko

    an elder emeritus

  • Active Members
  • PipPipPipPipPipPipPipPipPipPip
  • 3,361 posts
216
Excellent

Posted 22 July 2020 - 08:28 AM

Also, pay attention to the decimal point on absorbance readings (and on the extinction coefficient)


talent does what it can
genius does what it must
i used to do what i got paid to do





Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.