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Protein-protein-interaction


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#1 utopia

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Posted 02 October 2004 - 01:19 PM

hey folks,
can anybody help me please, which one of these methods is the most efficient one to showing an ínteraction bwn 2 proteins?

*Yeast 2-hybrid-system
*affinity chromatography
*co-immunopricipation
* ??or do you have a better idea?

Immunohistochemically, we could show the colocalization between 2 membranproteins. The question is now: do these proteins interact with each other directly and therefore we get a colocalisationsignal or they are just in close neighbourhood of each other and due to this adjacency we get a signal. :)

which method have I to use in order to be able to prove the colocalisation?

thank you in advance
Shokufeh

#2 jadefalcon

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Posted 03 October 2004 - 09:38 AM

Far-Westernblotting, perhaps?

mike
--- He who finds typos may keep them! ---

#3 Simonsays

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Posted 12 October 2004 - 05:42 AM

Hi
If you have the necessary antibodies to immunoprecipitate and blot, I would strongly suggest co-immunoprecipitation.

Simon :)

#4 TheMadScientist

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Posted 18 October 2004 - 01:35 PM

Does anyone know of any techniques to crosslink associated proteins for subsequent co-iping? I seem to remember a UV-crosslinking chemical for protein-protein interactions, separate from a DNA-protein UV crosslinking assay. Thanks ahead of time!




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