Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

How to statistically/bioinformatically measure the contribution of mutations to


  • Please log in to reply
1 reply to this topic

#1 mohsamir1984

mohsamir1984

    member

  • Active Members
  • Pip
  • 20 posts
0
Neutral

Posted 24 May 2020 - 02:57 PM

Dear All, 

I am stuck with one analyses. I have the frequency of occurence of several gene mutations in 50 bacterial isolates and these isolates were either resistant or senstive to certain drug. So the outcome is two phenotypes (resistant and senstive bacterial to certain drug).

 

I am searching for a statistical/bioinformatics analyses that could give a numerical estimate for the "importance" of each of these mutations in causing particular phenotype. In other words, how can one rank the mutations according to their contribution to inducing resistance to certain antimicrobial ? 

 

Example: I have the frequency of occurence of mutations A, B, C, D in 50 bacterial isolates (as 1: present, 01:L absent) and some of these isolates are resistant or senstive to certain drug. Which analyses is best for this estimate ? 

 

Thanks 



#2 bob1

bob1

    Thelymitra pulchella

  • Global Moderators
  • PipPipPipPipPipPipPipPipPipPip
  • 6,695 posts
567
Excellent

Posted 25 May 2020 - 07:07 PM

Principal component analysis? Depends on what sort of stats you have on each one - this is way beyond my understanding of stats, so you might want to talk to a proper statistician if you can.






Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.