Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

What is the relation between amplicon size and melting temp?


  • Please log in to reply
No replies to this topic

#1 Curtis

Curtis

    Metaller Scientist

  • Global Moderators
  • PipPipPipPipPipPipPipPipPipPip
  • 1,199 posts
63
Excellent

Posted 23 May 2020 - 09:11 AM

I am running HRM these days. One thing I never understood is the relation between the length of amplicon and melting temperature. I mean, will temperature increase if length is longer? I am guessing that the answer is negative. Probably melting temp does not work that way.

 

I have a set of primers that amplify a 120 bp amplicon. Then I have another set that amplify a 300 of the same gene. How different their melt peak should be? Definitely, it does not mean that the 300 bp amplicon must show a higher melting peak than that of the 120 bp? Correct? If the 120 bp product shows a peak at 81 C, it does not mean that the 300 bp product must be above 81 C? I have problem understanding this.






Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.